WP5 - Phenotyping, engineering and characterisation
OverviewObjectives: Identify, characterise and culture strains for microbiome therapeutics. Engineer microbial production of narrow spectrum antimicrobials for microbiome modulation. Engineer microbiota-residential bacterial strains for in situ production of health-beneficial compounds or degradation of harmful compounds. Validate best candidate strains based in vitro and ex vivo platforms such as artificial gut systems and stem cell derived mini gut systems.
SEEK ID: http://besttreat.hki-jena.de/projects/6
Public web page: Not specified
Organisms: No Organisms specified
BestTreat PALs: No PALs for this Project
Project start date: 1st Sep 2018
Project end date: 31st Aug 2022
Related items
Projects: WP1 - Coordination and Project Management, WP7 - Dissemination, Exploitation and Communication, WP8 - Open Research Data, WP6 - Network, Local and External Training, WP5 - Phenotyping, engineering and characterisation, WP7-Sub Project: Communication and outreach activities
Institutions: Technical University of Denmark (DTU)
Projects: WP1 - Coordination and Project Management, WP2 - Human cohorts and mice models, WP3 - Mechanistic studies of microbiome-host interactions, WP4 - Big data analysis of the gut microbiome, WP5 - Phenotyping, engineering and characterisation, WP6 - Network, Local and External Training, WP7 - Dissemination, Exploitation and Communication, WP8 - Open Research Data, WP7-Sub Project: Communication and outreach activities
Institutions: Academic Medical Centre Amsterdam
Projects: WP1 - Coordination and Project Management, WP7 - Dissemination, Exploitation and Communication, WP8 - Open Research Data, WP6 - Network, Local and External Training, WP5 - Phenotyping, engineering and characterisation, WP7-Sub Project: Communication and outreach activities
Institutions: University of Groningen (RUG)
https://orcid.org/0000-0002-9040-2681
Projects: WP1 - Coordination and Project Management, WP8 - Open Research Data, WP7 - Dissemination, Exploitation and Communication, WP6 - Network, Local and External Training, WP5 - Phenotyping, engineering and characterisation, WP7-Sub Project: Communication and outreach activities
Institutions: University of Groningen (RUG)
https://orcid.org/0000-0002-8078-9165
Projects: WP1 - Coordination and Project Management, WP5 - Phenotyping, engineering and characterisation, WP6 - Network, Local and External Training, WP7 - Dissemination, Exploitation and Communication, WP7-Sub Project: Communication and outreach activities
Institutions: Technical University of Denmark (DTU)
https://orcid.org/0000-0002-0829-2496
See “List of identified sterols, bile acids, and acylcarnitines” for details.
Figure 1: Separation of the different classes of standards (sterols, bile acids, and acylcarnitines) based on retention time in reversed-phase liquid chromatography in the positive ionization mode.
Figure 2: Extracted ion chromatograms of the base peak spectra of detected sterols acquired from reference “BACSMLS” standards in the positive ionization mode. Peak identities are as follows: (1) cortisone, (2) corticosterone,
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Creator: Howell Leung
Contributor: Howell Leung
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Objective: The aim of this study was to present and validate a sensitive and simple liquid chromatography–tandem mass spectrometry (LC–MS/MS) method to detect the presence and distribution of NAFLD-related metabolites (sterols, bile acids, and acylcarnitines) in biological samples. These compound classes were considered as they are implicated in the development and progression of NAFLD; their detection by MS-based analytics is challenging due to the occurrence of many isomers and stereoisomers;
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Creator: Howell Leung
Contributor: Howell Leung
Abstract (Expand)
Authors: S. Lopez-Escalera, A. Wellejus
Date Published: 25th Jul 2022
Journal: Biochem Biophys Rep
Abstract (Expand)
Authors: Alex Hedin, V. E. Rees, H. Zhang, V. Kruse, R. Vazquez-Uribe, M. O. A. Sommer
Date Published: 25th May 2022
Journal: Sci Rep
